Ribosomal protein S15



Ribosomal protein S15 (RPS15) is one of the 22 proteins which belong to the small subunit of the bacterial ribosome. It is involved in the early steps of ribosome assembly. The images at the left and at the right correspond to one representative RPS15, i.e. the crystal structure of Ribosomal protein S15 from Thermus thermophilus (2zm6, chain O).

3D Structures of Ribosomal protein S15
Update June 2011

S15
1a32 – RPS15 – Geobacillus stearothermophilus

1ab3, 2fkx - TtRPS15 – Thermus thermophilus - NMR

1kuq, 1f7y, 1dk1 - TtRPS15+16S RRNA fragment

2vaz – EcRPS15+RPSO mRNA operator – Escherichia coli – Cryo-EM

S15 in small ribosomal subunit
2zm6, 1n36, 1j5e, 3oto- TtRPS15 in 30S ribosomal subunit+THX+16S RRNA

2vqe, 2vqf, 2uuc, 2e5l, 2uu9, 2uua, 2uub, 3pyn, 3pyq, 3pys,  3pyu - TtRPS15 in 30S ribosomal subunit+16S RRNA+RNA fragment

3i8g, 3i8h - TtRPS15 in 30S ribosomal subunit+16S RRNA+mRNA+tRNA-Phe

3kc4 – EcRPS15 in 30S ribosomal subunit

3i1m, 3i1o, 3df1, 3df3, 2vho, 2vhp, 2avy, 2aw7, 1pnx, 3ofo, 3ofp, 3or9, 3ora, 3kc4 - EcRPS15 in 30S ribosomal subunit+16S RRNA

1i94, 1fka - TtRPS15 in 30S ribosomal subunit+16S RRNA

1p87, 1p6g - EcRPS15 in 30S ribosomal subunit+16S RRNA – Cryo-EM

3bbn - RPS15 in 30S ribosomal subunit+16S RRNA – Cryo-EM – spinach

2zkq – RPS15 in 40S ribosomal subunit+18S RRNA - dog

S15 in small + large ribosomal subunit
1ml5, 1eg0 - EcRPS15 in 30S ribosomal subunit+50S ribosomal subunit+THX+chain release factor 2+P-site tRNA+tRNA Phe+mRNA fragment+23S RRNA+5S RRNA+16S RRNA - EM

S15 in small ribosomal subunit+ chain release factor
3mr8, 3ms0 – TtRPS15 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+mRNA+16S RRNA

3d5a, 3d5c, 3f1e, 3f1g - TtRPS15 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+E-site tRNA+mRNA +16S RRNA

2b64 - TtRPS15 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 1

2b9m - TtRPS15 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 2

2x9r, 2x9t, 2wh1, 2wh3 - TtRPS15 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+mRNA +16S RRNA

3f1e, 3f1g - TtRPS15 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+E-site tRNA +16S RRNA

S15 in small ribosomal subunit+tRNA+mRNA
2hgi - TtRPS15 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet

2hgp - TtRPS15 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA

2i2p, 2i2u - EcRPS15 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA

2hgr - TtRPS15 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet+mRNA

1yl4 – TtRPS15 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+THRS mRNA operator

1pns - EcRPS15 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+mRNA

1xnq, 1xnr - TtRPS15 in 30S ribosomal subunit+THX+mRNA+tRNA anticodon+16S RRNA

S15 in small ribosomal subunit+A-site tRNA
2uxd, 2uxc - TtRPS15 in 30S ribosomal subunit+THX+A-site mRNA CGGG+tRNA anticodon+16S RRNA

1n32, 1n33 - TtRPS15 in 30S ribosomal subunit+THX+A-site mRNA fragment+tRNA anticodon+16S RRNA

1n34 - TtRPS15 in 30S ribosomal subunit+THX+A-site mRNA fragment +16S RRNA

1xmo, 1xmq - TtRPS15 in 30S ribosomal subunit+THX+A-site mRNA+tRNA anticodon+16S RRNA

2uxb - TtRPS15 in 30S ribosomal subunit+THX+A-site mRNA GGGU+tRNA anticodon+16S RRNA

S15 in small ribosomal subunit+P-site tRNA
3i8g - TtRPS15 in 30S ribosomal subunit+P-site tRNA+mRNA +16S RRNA

3i8h, 3i9b, 3i9d - TtRPS15 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA

1ibk - TtRPS15 in 30S ribosomal subunit+THX+P-site mRNA fragment +16S RRNA

2wwl - EcRPS15 in 30S ribosomal subunit+Poly-Ala chain+P-site tRNA+mRNA +16S RRNA - Escherichia coli

3i1q - EcRPS15 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA

3i1s, 3i1z, 3i21 - EcRPS15 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA +16S RNA

S15 in small ribosomal subunit+A-site tRNA+P-site tRNA
1ibl, 1ibm - TtRPS15 in 30S ribosomal subunit+THX+A-site mRNA fragment+P-site mRNA fragment+tRNA anticodon+16S RRNA

S15 in small ribosomal subunit+P-site tRNA+E-site tRNA
2ow8 - TtRPS15 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA+mRNA +16S RRNA

2b9o - TtRPS15 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA

2qnh - TtRPS15 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA

S15 in small ribosomal subunit+A-site tRNA+P-site tRNA+E-site tRNA
2gy9, 2gyb - EcRPS15 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA – Cryo-EM

1jgo, 1jgp, 1jgq, 1gix - TtRPS15 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+tRNA Phe+mRNA +16S RRNA

3e1a, 3e1c - EcRPS15 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA

S15 in small ribosomal subunit + antibiotics
1i95, 1i97, 2hhh,  2f4v, 3oge, 3ogy, 3ohc, 3ohd, 3ohy, 3oi0, 3oi2, 3oi4-  TtRPS15 in 30S ribosomal subunit+16S RRNA+antibiotic

1hnw, 1hnx, 1hnz,  1fjg - TtRPS15 in 30S ribosomal subunit+16S RRNA+mRNA fragment+antibiotic

2qbb, 2qb9, 1vs5, 1vs7,  2qan,  2qal,  2qou,  2qow,  2qoy,  2qp0,  2z4k,  2z4m, 3oaq, 3oar, 3ofa, 3ofb, 3ofo, 3ofp, 3ofx, 3ofy - EcRPS15 in 30S ribosomal subunit+16S RRNA+antibiotic

2j00, 2j02 - TtRPS15 in 30S ribosomal subunit+16S RRNA+paromomycin+P-site tRNA fMet+E-site tRNA Phe+mRNA

2wdg, 2wdh, 2wdk, 2wdm - TtRPS15 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA+tRNA Phe+paromomycin

3knj, 3knh - TtRPS15 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+viomycin

3knl, 3knn - TtRPS15 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+capreomycin

S15 in small ribosomal subunit + ribosome recycling factor
2qbd, 2qbf – EcRPS15 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor

3r8n, 3r8o – EcRPS15 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor + mRNA + tRNA

2qbh, 2qbj - EcRPS15 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+gentamicin

2v46, 2v48 - TtRPS15 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+P-site tRNA Phe+mRNA+tRNA fMet

S15 in small ribosomal subunit + elongation factor
2wri, 2wrk - TtRPS15 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA+elongation factor G

2wrn, 2wrq - TtRPS15 in 30S ribosomal subunit+THX+A-site tRNA+mRNA +16S RRNA+elongation factor TU-4

3fic - TtRPS15 in 30S ribosomal subunit+THX+A-site tRNA+P-site tRNA+E-site tRNA+mRNA codon+16S RRNA+elongation factor TU-A

3izv – EcRPS15 in 30S ribosomal subunit+A/T-site tRNA Phe +16S RRNA+elongation factor TU

3izw – EcRPS15 in 30S ribosomal subunit+A-site tRNA Phe +mRNA +16S RRNA+elongation factor TU

2y0u, 2y0w,  2y0y,  2y10,  2y12,  2y14,  2y16,  2y18 - TtRPS15 in 30S subunit+THX+A-site tRNA+E-site tRMA+mRNA codon+16S RRNA+elongation factor TU

3fih - EcRPS15 in 30S ribosomal subunit+A-site tRNA Phe+P-site tRNA+mRNA +16S RRNA+elongation factor TU

3huw, 3huy - TtRPS15 in 30S ribosomal subunit+THX+tRNA-Met+mRNA +16S RRNA+elongation factor P

1s1h - yRPS15 in 40S ribosomal subunit+18S RRNA+elongation factor 2 – Cryo-EM – yeast

S15 in small ribosomal subunit + translation initiation factor
1i96 - TtRPS15 in 30S ribosomal subunit+THX+IF3+16S RRNA

1hr0 - TtRPS15 in 30S ribosomal subunit+THX+IF+16S RRNA

S15 in small ribosomal subunit + various proteins
3kiq, 3kis, 3kiu, 3kix - TtRPS15 in 30S ribosomal subunit+toxin RelE+16S RRNA

1voq, 1vos, 1vov, 1vox, 1voz - EcRPS15 in 30S ribosomal subunit+protein Y+16S RRNA

2xfz, 2xg1 - TtRPS15 in 30S ribosomal subunit+tRNA Phe+mRNA+colicin E3+16S RRNA

3jyv - RPS15+RPS0+RPS2+RPS3+RPS5+RPS9+RPS11+RPS13+RPS14+RPS16+RPS18+RPS20+RPS22+RPS23+RPS29+18S RRNA+RACK1+S19E+tRNA – Cryo-EM - Thermomyces lanuginosus

S15 in small 40S ribosomal subunit + various proteins
3iz6 - RPS15 in 40S ribosomal subunit +RACK1+18S RRNA – wheat – Cryo-EM

2o2z, 3o30 - yRPS15 in 40S ribosomal subunit +RACK1

S15 in part of small ribosomal subunit
3iy8 - EcRPS15+EcRPS5+EcRPS6+EcRPS9+EcRPS9 EcRPS8+EcRPS11+EcRPS12+EcRPS16+EcRPS17+EcRPS18+Leishmania taretolae mRNA

1g1x - TtRPS15+TtRPS6+TtRPS18+16S RRNA fragment

1qd7 – TtRPS15+TtRPS4+TtRPS5+TtRPS7+TtRPS8+TtRPS6+TtRPS17+TtRPS20+16S RRNA fragment